FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34832778
Sequences flagged as poor quality0
Sequence length35-42
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG54700.09.4624355
GTATAGA42600.09.2411221
GTATAGG32550.09.043541
GTGTAGG62850.08.9722871
TAGACTG56100.08.6891485
GTGTAGA58100.08.4544431
GTACTAG24300.08.3191691
TACACTG72250.08.3170485
GTCTAGA50950.08.28350451
ATACCGT9450.08.2533466
TAGACCG12650.08.1272735
CTATACT48100.08.1073474
GGTATAC22900.08.0533991
CTAGGAC63200.08.0494113
GTATATA101600.08.0461581
GTATTAG45700.07.9934151
ATACTGT75050.07.95954856
TCTATAC36350.07.94845063
CTAATAC41100.07.93551833
TAGTACT33900.07.79090174
Produced by FastQC (version 0.11.5)