FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34832778
Sequences flagged as poor quality0
Sequence length35-42
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG58350.09.6298425
TAGACTG53450.08.8545065
TACACCG14300.08.8008185
TAGACCG13600.08.7324615
CTAGGAC59850.08.7072843
GTGTAGG69450.08.6788271
GTATAGA40500.08.6669151
TAGGACT58300.08.2395014
TACACTG68550.08.2228135
CTAGACT53950.08.2138864
GTATAGG32900.08.0825751
CCTATAC27000.08.0749693
GTATTAG47150.08.0461191
TCTAGAC44450.07.97549723
GTCCTAG68950.07.89329771
GTGTTAG61800.07.859891
ATACTGT75250.07.77334936
GTAAGAC44550.07.71886633
GTGTAGA59350.07.70643231
GTATATA97650.07.6430071
Produced by FastQC (version 0.11.5)