FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57501613
Sequences flagged as poor quality0
Sequence length35-42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG96200.011.6028975
CTAGGAC109300.09.3693083
ATACTGT127850.09.3680586
TACACTG118800.09.0973385
TAGACTG97300.09.0863335
TAGACAG134700.08.9179095
TCTATAC62900.08.872783
TAGGACC65600.08.8586964
GTATAGA69250.08.853941
CTATACT88100.08.808424
GTATTAG78200.08.7243741
GTATATA167100.08.6111251
TAGGACT110050.08.5165844
CTAGACT93000.08.4967294
TCTAGAC78250.08.4454793
TACACCG24400.08.4230455
GTATAGG58250.08.30514051
AGGACTG193800.08.173065
TCTACAC86750.08.1081323
GTACTAG39450.08.085481
Produced by FastQC (version 0.11.5)