FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57501613
Sequences flagged as poor quality0
Sequence length35-42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG94200.011.1896585
TACACTG114350.010.1164415
TCTATAC62450.010.0418483
TAGACTG96650.09.4029975
GTATAGA68150.09.3079011
GTATAGG54600.09.1190221
ATACTGT128000.09.0099926
TAGACAG130750.08.9152795
CTACACT112450.08.8063574
CTATACT84350.08.77884
TCTACAC88200.08.5562573
GTATTAG79800.08.4819381
GTGTAGG116650.08.3999171
TAATACT91250.08.3673584
CTAGGAC109250.08.3345953
CCTATAC52850.08.3128413
CTAGACT93700.08.2998244
TAGGACC63900.08.1783394
GTAGGAC61550.08.0876263
TAGGACT105500.08.0263744
Produced by FastQC (version 0.11.5)