FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48027616
Sequences flagged as poor quality0
Sequence length35-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG79900.011.163455
TCTATAC57950.010.6488033
TAGACTG85400.010.1122635
TACACTG108350.09.9179285
CTATACT90600.09.8645824
CGTGCGA17950.09.58232610
ATACTGT111200.09.3926416
AGGACTG166750.09.1774545
TAGACAG107750.09.0515435
TAGACCG14900.09.0449155
GGACTGT111400.08.9778256
TAGTACT55600.08.9301664
CTACACT108550.08.9031364
GTATAGG55150.08.8784111
CTAGGAC100200.08.8309573
CTAGACT87300.08.7952864
TACACCG21400.08.7835345
GTATACG12150.08.7608591
GTGTAGG100950.08.7341721
CTTAGAC55950.08.7158073
Produced by FastQC (version 0.11.5)