FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44642716
Sequences flagged as poor quality0
Sequence length35-42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG90450.011.6634125
TACACTG101750.09.8984185
TACACCG19050.09.8491525
TAGACAG112950.09.5907625
ATACTGT116350.09.5235286
GTATAGG44450.09.3974221
CTATACT69000.09.2607274
TAGGACT82900.09.159884
TAGACTG76300.09.1402835
TCTATAC52800.09.0177273
CTAGGAC82000.08.9148683
GTACTAG36550.08.9104361
GTATTAG65200.08.6870211
CTAGACT72700.08.6676734
CTACACT88500.08.6002714
TCTACAC68100.08.55120853
CTAGACA102150.08.4907244
GTCTAGG81400.08.4585071
CCTATAC42600.08.4346123
GTGTAGG82750.08.3846821
Produced by FastQC (version 0.11.5)