FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44642716
Sequences flagged as poor quality0
Sequence length35-42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG87950.011.5640695
ATACTGT109550.09.703966
TAGACTG77500.09.5401075
TCTATAC51800.09.2190653
CTAGGAC80750.09.1994023
TACACTG99800.09.1807445
CTATACT70100.09.0329584
TAGACAG108600.08.9417225
GTATAGA60150.08.8241881
CTAGACT75350.08.7791684
GTGTAGG81250.08.6883761
CTACACT89950.08.6716634
TCTAGAC65900.08.642183
GTGTTAG83150.08.6175131
TCTACAC73700.08.5674893
GTATATA161350.08.5529271
TAGGACT81500.08.4639294
TTATACT79650.08.3718344
GTTCTAG85800.08.3513541
GTATAGG46300.08.3303771
Produced by FastQC (version 0.11.5)