FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50739899
Sequences flagged as poor quality0
Sequence length35-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG97950.011.2282355
TACACCG19400.010.0418865
TACACTG111600.09.7120655
GTATAGA68750.09.4537391
TAGACTG87100.09.3532835
TAGACAG130850.09.0547555
ATACTGT130150.08.9809616
TCTACAC77600.08.6267313
CTAGGAC85850.08.6228773
GTGTAGA84550.08.6087181
TCTATAC59450.08.549613
GTACTAG38150.08.4950671
CTAGACT83450.08.4889794
TAGTACT53050.08.4461144
GTCTAGA79350.08.3917241
CTACACT98600.08.3880644
GTATATA181150.08.3782391
GTATAGG48650.08.3725011
CCTATAC47000.08.3274243
GTATAAG60850.08.3236871
Produced by FastQC (version 0.11.5)