FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50739899
Sequences flagged as poor quality0
Sequence length35-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG93500.010.7893095
TACACTG110250.09.9367055
TAGACTG89300.09.1563185
CTACACT98750.09.0505194
GTATAGA69050.08.9727931
TACACCG17950.08.8737755
TAGACAG123500.08.8132985
ATACTGT129050.08.7499556
GTATAGG50250.08.7365281
GTGTAGG90350.08.6599891
GTGTTAG93450.08.5242561
TCTATAC62100.08.5209013
CTAGGAC86850.08.49712853
GTCCTAC50950.08.4775221
TCTACAC75400.08.4026773
CCTATAC42700.08.3720043
CTATACT78450.08.3018344
GTATAAG61500.08.2897351
GTCCTAG96750.08.2335061
GTCTTAG80000.08.2314471
Produced by FastQC (version 0.11.5)