FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42723302
Sequences flagged as poor quality0
Sequence length35-42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG72200.011.6757095
TCTATAC49950.010.6233523
CTATACT72950.010.2906174
TACACTG96600.010.0125865
GTATAGA58250.09.9327591
ATACTGT102600.09.9287126
TAGACTG74700.09.8119735
GTATAGG47300.09.4555681
TAGACCG14400.09.3665135
GTATAAG50450.09.2521521
AGGACTG137100.09.2295165
CCTATAC46800.09.0631243
TAGACAG100700.08.9880935
CTAGTAC28450.08.9203033
GTCCTAG87750.08.8588061
CTAGGAC80250.08.8458753
GTACTAG31100.08.8454421
CTACACT95000.08.8361664
CTAGCCT100300.08.7939064
GGACTGT96450.08.7217676
Produced by FastQC (version 0.11.5)