FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57426614
Sequences flagged as poor quality0
Sequence length35-42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG214400.014.1851425
ATACTGT259100.012.1492936
TCTATAC109250.011.6425213
GTATAGA118200.011.6232981
TACACTG174600.011.4767685
CTATACT133450.011.0887224
TAGACAG190100.011.008245
CTAGGAC93950.010.7967933
ATACCGT19350.010.55775456
TAGACTG127500.010.4497485
TAGGACT110150.010.2087914
CTAATAC117100.010.1793753
TATACCG15900.010.1671455
ATATACT261800.010.1579774
GTATTAG113950.010.1383281
GTATAGG82750.010.1377441
CTACACT126900.010.1211944
GTATATA408850.09.9375541
CTAGACT105100.09.8710934
ACTGTAC205550.09.7993868
Produced by FastQC (version 0.11.5)