FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57426614
Sequences flagged as poor quality0
Sequence length35-42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG212750.014.4257125
ATACTGT253550.012.1750086
TACACTG169950.012.1506485
TAGACAG187700.011.3897925
CTATACT137350.011.229984
GTATAGA121550.011.1981981
TCTATAC114000.011.1605193
TAGACTG122800.011.1141785
CTAGACT103200.010.5380354
CCTATAC78000.010.3883483
ACTGTAC196600.010.3147038
TCTAGAC100500.010.3081023
GTATAGG79550.010.1412141
GTGTAGG108650.010.1072511
ACTGATC114750.09.9899118
ATATACT258450.09.9696394
GTATTAG114500.09.9322771
CTAGGAC92450.09.8602223
TAGGACT108400.09.8358054
CCTACAC100150.09.8295813
Produced by FastQC (version 0.11.5)