FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41948724
Sequences flagged as poor quality0
Sequence length35-42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1152440.2747258772400324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA13350.012.21837510
TATACTG79400.011.875675
GTATAGA64850.010.6288561
TCTATAC60750.010.5013
ATACTGT103400.010.0113256
TACACTG92250.09.4913415
TAGACAG110650.09.2745785
CTATACT81950.09.2561554
CTAATAC79200.09.1958343
TAGTCCG22650.08.999055
TCTACAC72750.08.963743
GTGTAGA78100.08.9390651
CCGTGCG27450.08.9130439
CTACACT89100.08.9111994
TAGACTG75750.08.7509655
GTGTAGG83800.08.7116431
GTATTAG81200.08.7069041
TAGACCG11850.08.6751435
GTATATA155150.08.668361
TATACAG77900.08.6232065
Produced by FastQC (version 0.11.5)