FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29929752
Sequences flagged as poor quality0
Sequence length35-42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN807260.26971823889486285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG56700.012.4194785
CGTGCGA10250.011.2511510
GTATAGA45750.010.8523471
TCTATAC40850.010.6360133
TAGACAG77050.010.1292195
CTATACT55450.09.8515934
CCGTGCG22550.09.6773519
ATACTGT77550.09.6528856
CTTAGAC35450.09.3547073
CTAGTAC19950.09.3336443
TAGACTG54750.09.2980895
GTATAGG34450.09.2648841
CCTATAC34500.09.2010783
TACACTG68250.09.1222175
TCTACAC52500.09.1203033
CCTACAC59350.09.0537143
GTGTAGA55400.08.9940081
TTAGACT46400.08.9827064
CTAAGAC52400.08.9684923
CTAGGAC55850.08.9542823
Produced by FastQC (version 0.11.5)