FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46606332
Sequences flagged as poor quality0
Sequence length35-42
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG117350.012.1800815
TCTATAC66300.010.33744053
TAGACTG89150.010.0180695
CTATACT91800.09.9803614
ATACTGT154200.09.8911366
TACACTG111550.09.5662625
TAGACAG136650.09.4982835
GTATAGA78750.09.4972791
GTGTAGG89100.09.3909791
CCTATAC54350.09.3434173
GTATAGG56250.09.0641491
CTAGGAC79700.08.8444623
CTAGACT79350.08.8163444
GTATTAG81950.08.7795791
GTCCTAG88800.08.7625161
GTGTAGA86500.08.7079791
CTACACT99250.08.6766184
TCTAGAC74300.08.6747523
CCTACAC87000.08.5717463
GTACTAG37700.08.4820111
Produced by FastQC (version 0.11.5)