FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46606332
Sequences flagged as poor quality0
Sequence length35-42
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG114350.011.4484985
ATACTGT145350.010.0215666
GTGTAGG88950.010.0039421
GTATAGA77850.09.559491
TACACTG115700.09.4418915
GTACTAG39950.09.4253711
TAGACAG131250.09.3382825
TCTATAC72800.09.3200533
GTATAGG57700.09.2655261
CTACACT101750.09.1123894
TAGACTG88100.08.9924115
CTATACT90000.08.8613524
CTAGGAC82050.08.8321813
TAGGACT91800.08.8230424
GTATTAG84100.08.7223481
CCTATAC51300.08.6904593
CTAATAC80800.08.5511523
TCTACAC78150.08.5229243
TAATACT103800.08.5046264
GTATATA230100.08.49865151
Produced by FastQC (version 0.11.5)