FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43069988
Sequences flagged as poor quality0
Sequence length35-42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG95600.011.0661275
TACACTG98400.09.67071155
TAGACTG76700.09.4956715
TAGACAG119200.09.35090355
ATACTGT119800.09.0674336
GTGTAGG81800.08.7516441
CTATACT72900.08.7022334
TCTATAC56600.08.6722633
GTATAGA65700.08.6307041
CCTATAC45000.08.6238413
GTATAGG49550.08.4755181
GTACTAG35550.08.4736581
TAAGACT78750.08.4383184
GTATTAG68400.08.3936431
CTAGGAC71100.08.2993443
CTACACT83050.08.1721124
GTAAGAC61150.08.1428993
GTGTAGA72550.08.133331
GTGTTAG85250.08.0856851
GTATAAG64150.08.0658061
Produced by FastQC (version 0.11.5)