FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32917952
Sequences flagged as poor quality0
Sequence length35-42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG57650.010.7875625
TAGACTG56500.010.1604295
TACACTG74650.09.5651265
CTAGGAC56250.09.3862923
GTACTAG26250.09.3156841
TCTATAC37100.09.3123873
TAGACAG75200.09.2831175
ATACTGT79850.09.2750636
GTATAGG32300.09.2714821
GTATAGA40100.09.1914471
CTATACT46150.09.0218954
CTAGACT58950.09.0165234
CCTACAC58800.08.918973
CTAGTAC24700.08.8945723
TAGGACC38250.08.8008344
TACACCG14300.08.7970665
TAGGACT59850.08.7329214
GTCTAGG57000.08.7045861
TCTACAC51150.08.6941763
TCTAGAC46400.08.5914183
Produced by FastQC (version 0.11.5)