FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62405201
Sequences flagged as poor quality0
Sequence length35-42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG118550.010.562315
TACACTG126950.09.7518185
ATACTGT146400.09.291046
TAGACTG99600.09.1933185
TCTATAC71000.09.1795963
CCTATAC56750.09.1439923
TAGACAG153200.09.1329155
GTATAGA78150.09.1121611
CTATACT93200.09.0073684
CTAGGAC110850.08.8353453
GTATAGG63050.08.6534611
CTACACT119900.08.6411634
TACACCG23600.08.5552745
TAATACT100000.08.5011324
TACACAG158900.08.426345
GTATTAG85000.08.3986671
GTGTAGG108750.08.323691
GTGTTAG119450.08.31957151
GTCTTAG98850.08.3150631
GTATATA203900.08.3141721
Produced by FastQC (version 0.11.5)