FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10670138
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264470.24785996207359268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20950.051.12114346
TCGTATG20550.047.34762243
CGTATGC23300.045.8151544
GTCGTAT13450.043.71718642
CGTCTGA75900.039.6123716
ACACGTC79650.038.1862813
ACGTCTG78750.038.0459815
GTATGCC30900.037.37839545
CACGTCT84150.036.06205714
CACACGT88200.034.32597412
CTCGTAT18750.033.03978342
GTCACAC92450.032.10347729
GCACACG101650.029.43988611
CTGAACT105150.029.02555719
ATGCCGT34100.028.84104747
TCTGAAC107650.028.4165118
CAGTCAC104650.028.29386127
ACAGTGA99500.028.28148734
ACACAGT107650.027.99357832
GTCTGAA108950.027.8846917
Produced by FastQC (version 0.11.5)