FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10670138
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129430.12130114905730366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT15000.016.33324849
CCGTATG11450.016.20124211
CGTATGG12350.015.87073412
ACCGTAT12700.015.70902110
GTACGCA17900.014.27346850
GTCTAAC13350.013.8998141
CGACCGT7750.013.5529541
GTAGGTC13600.012.86794211
TACCGTA15900.012.7675889
TCTCGAT20150.012.67995348
CGGTACA19600.012.67737761
TTCTCGA20200.012.64856747
AGTACGC21250.012.51758249
ATGCGTG29600.012.4186182
ATTCCGT18750.012.133153
TACGCAC19600.011.96405551
TCGATTC20450.011.80906350
GATGCGT30350.011.7667311
ACGCACG20700.011.66644252
CCGGTAC20000.011.5490260
Produced by FastQC (version 0.11.5)