FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20331029
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA547090.26909115126440475No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT244910.12046119259384264TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46350.054.2921248
TCGTATG47650.053.2522745
CGTATGC51450.049.93102646
TGAAACG61650.042.9202735
GAAACGA59100.042.04771836
CTCGTAT55500.041.3688944
CGTGAAA65150.040.9905633
GTATGCC66350.038.45424347
AACGATC57100.037.0224338
AGTCACG74900.036.49508328
AAACGAT66550.036.44630437
GTCACGT74650.036.43012229
ATGCCGT70600.035.44532849
ACGTGAA78100.034.59720632
CACGTGA79350.034.3167731
ACGATCT62950.033.3596739
TCACGTG84000.032.50026330
GCCGTCT78050.030.31325151
CGTCTGA95200.029.00730116
ATCTCGT72250.028.82319542
Produced by FastQC (version 0.11.5)