FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28323161
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG43850.020.67283611
CGTATGG44100.020.6350112
ACCGTAT47700.019.44452910
TACCGTA52550.018.5157459
CTACCGT57100.017.4694568
GCGCTAA61500.014.22855742
ACTACCG69700.013.80921657
CGCTAAG66850.013.08977943
CCGTCAT72650.012.4334391
GCGATAG62150.012.38825266
CGACCGT25500.011.9450031
CGATAGA67250.011.70897167
AGCGCTA76700.011.6826741
CGACCTA21150.011.4189924
TAGGCGA69700.011.14676163
GGCGATA67700.011.06250965
ATAGGCG67350.011.01609162
GTAGGTC39750.010.56608311
CGGTACA34850.010.24300661
GTATGGC90950.010.00550513
Produced by FastQC (version 0.11.5)