FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19321402
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA446520.23110124203202229No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC210240.10881197958616047TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG45600.052.95994643
TATGCCG43900.052.3794346
CGTATGC51300.046.1885844
CTCGTAT46900.042.0144642
ACGTCTG115400.037.88155415
GTATGCC64550.037.79179845
CGTCTGA118250.036.90907316
ACACGTC122800.035.8836213
CACGTCT131350.033.4948114
ATGCCGT71150.032.31835647
GTCGTAT20100.031.86532442
CACACGT138000.031.75376912
TCTCGTA64950.031.0928941
AATCTCG71100.030.3235539
ATCTCGT69600.030.07166740
CCTTGTA141250.029.88370933
CTTGTAA144600.028.3234
GCACACG157250.027.82242811
ACCTTGT150550.027.80513232
GTCACCT154150.027.33713329
Produced by FastQC (version 0.11.5)