FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478934
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472120.1601550449551534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390260.13238606253536847No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321050.10890828006195884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27200.043.87631648
TCGTATG29500.039.86260245
TCCGTAT38700.030.65939737
CGTATGC40850.029.30089846
TATCTCG43350.028.17668241
CGTATCT44550.026.16158339
CTCGTAT45450.025.71958244
CCCCCCC720450.024.2989711
GTATGCC55900.021.5999147
GTATCGA43800.021.1001974
TTCCGTA63600.019.53660436
CCCCCCT415800.019.2126753
CGTATCG54150.018.554113
CCCGTAT57000.018.3120461
TATCGAA51400.018.252755
GTTACGA24300.018.1518524
ACGACTT63700.018.1455421
TCGACCG81350.017.16747531
GTTCGAC82000.017.11693629
TTACGAC24500.016.8605845
Produced by FastQC (version 0.11.5)