FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478934
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT24200.021.1489451
CTCGATT87950.019.18098449
TTCTCGA93800.018.05935347
ATTCCGT94800.017.94226653
TCTCGAT96600.017.49966448
GTCTAAC33750.017.449651
CATATAG81650.017.35955612
GTACGCA110250.017.23773650
CCAGTAG112950.017.1670825
ACCGTTG29200.017.0283093
ACGCACG112150.017.0077452
CATATGC120900.016.61595712
TTTCTCG102200.016.57502446
ACGGACA104150.016.49916818
TACGCAC114250.016.48077651
CGGTACA116100.016.36855761
ACACGGA108350.016.02110716
CCGGTAC120350.015.79052260
TAGCATA127950.015.6730729
GCCGGTA122900.015.66228259
Produced by FastQC (version 0.11.5)