FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29103362
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG299610.10294686916240124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT113750.020.33792949
GTACGCA118450.020.06279450
TACGCAC119250.019.89857751
TAGCATA127350.019.8141699
ATTCCGT115950.019.61967753
CCAGTAG124600.019.5788465
TCTCGAT118300.019.52611748
ACGCACG123850.019.41380552
TTCTCGA121950.019.08525847
CCGGTAC125500.018.96306260
GTAGCAT133400.018.9417848
CGGTACA126300.018.8152761
AGTACGC127200.018.7377249
ACGGACA127100.018.61404218
CATATGC133900.018.40058512
CGACCGT23000.017.984451
GCCGGTA134300.017.69435759
ACACGGA133650.017.59705216
GCATATG142550.017.48043811
TAAGTAC139950.017.23076447
Produced by FastQC (version 0.11.5)