FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28891757
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA27900.016.5624834
CATATAG77000.016.40811312
GTCGTAA17000.013.5883895
GCGCTAA40250.013.39078442
CGACCGT20950.013.3864831
ACCGTAT33900.013.00807410
GTCTAAC33050.012.9404361
GTACGCA52000.012.78809850
GCGATAG47000.012.43555866
CGGTACA53850.012.41345961
ACGCACG54350.012.29938852
CCGTATG36100.012.11839111
CGGCGAA36650.012.0519441
TCATATA110150.012.04195111
GCGAACG33300.011.9874923
ATAGGCG51200.011.89390562
CCGGTAC56250.011.88381960
GCCGGTA56050.011.86376159
TACCGTA41150.011.7368479
GCCTATC106350.011.6825486
Produced by FastQC (version 0.11.5)