FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30095863
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT81500.020.6187111
GTTACGA28500.019.5312884
CGAGCGA56600.018.85983753
TTACGAC29450.018.6635885
TGTTACG30350.017.8793163
GTTCGAC111300.017.86107829
TCGACCG112450.017.49167331
CGACTTT116700.017.4594272
CGACCGT113300.017.39133632
GAGCGAC63300.016.5318754
AAGTTCG125600.016.27340527
GTATCGA42800.016.032124
TTCGACC126450.015.61042630
CGTATCG48800.015.4957223
GACCGTC129900.015.30360433
TATCGAA49150.014.9581045
GCCGACC131100.014.78977666
AGTTCGA139400.014.46152928
CTAGATA144350.014.40230717
ACCCCGG140850.014.28780570
Produced by FastQC (version 0.11.5)