FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38625036
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG401030.10382644044655388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG34050.013.77344862
CCCCCCC392000.013.4946161
CTATCGC33550.013.45712363
CGCCTAT40000.011.81214667
CCCCCCT211000.011.4983093
TATCGCC39950.011.38889464
CTCCGTT86700.011.0236551
CCCGTAT54300.010.5736791
CTCCGAT77050.010.4959421
GTTCGAC37950.010.23684729
TCGACCG38150.010.1831831
CGTATCG38550.09.9896783
CGCGTTA25200.09.721930534
CCCGAAT84800.09.6605551
CTCGTTT72250.09.5942141
TCGCCTA48900.09.51914166
CTCGGTT75550.09.3604981
CTCGTAT40050.09.3532681
CCGTATC53700.09.3227452
ATACGCG12400.09.31423643
Produced by FastQC (version 0.11.5)