FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38625036
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG75350.013.8882566
CGATAGA78800.013.28019867
ATAGGCG81650.012.98811262
GGCGATA82900.012.62339865
TAGGCGA92950.011.93629263
GTATAGG98750.011.19978160
TAGGTCG22900.010.8514017
CGACCGT33900.010.744251
TACCGTA36250.010.6205249
ACCGTAT35600.010.42117210
CATATAG68500.010.167685512
CGACCTA29500.010.08531954
GCGAACG31950.09.9704043
CGCACGA33750.09.85166128
TAATTCG23100.09.84829315
AGTATAG119350.09.47195759
GTCTAAC41850.09.4563911
ACCTTAC126200.09.37382715
ACCTTAG124550.09.18890231
TATAGGC118200.09.11995861
Produced by FastQC (version 0.11.5)