FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37992668
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425830.11208215227211736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC428750.017.5227361
CGAGCGA60650.016.79259953
GTTCGAC91050.016.75954629
TCGACCG89950.016.73104131
CGACCGT91550.016.43863932
ACGACTT85100.015.7559841
AAGTTCG103400.015.02860627
TGTTACG30550.014.897333
GAGCGAC69450.014.765654
CCCCCCT229500.014.4611213
GTTACGA32100.014.3961314
TTACGAC32200.014.3515185
TTCGACC107700.014.20108330
CGACTTT111950.014.0410182
GACCGTC110900.013.94910733
ACGTTCG54500.013.2328486
GTATCGA39000.012.9263154
GCGATCG58900.012.53784641
CGCGTTA26300.012.509150534
CGTTCGA60500.012.4379257
Produced by FastQC (version 0.11.5)