FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37992668
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC50400.015.7738721
CGCACGA49400.015.72843428
ACGCACG85350.015.04949752
CGACCTA29650.014.2843024
GTACGCA90150.014.131721550
CCGGTAC93600.013.90998260
TACGCAC92950.013.89429151
TAGCATA102050.013.8899939
GCGATAG72550.013.65238266
TAACACG57500.013.6357334
TGCGCGA59450.013.59939511
CGGTACA95900.013.57637261
GCCGGTA95500.013.44999459
ACCGTAT48200.013.43334410
ATAGGCG74750.013.29739562
AACACGT61800.013.0262165
GCGCTAA57000.012.95588342
AGTACGC103000.012.91236549
CGATAGA77950.012.796411567
CGCGAGT61950.012.76810913
Produced by FastQC (version 0.11.5)