FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39857433
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG452800.11360490777215884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC144400.021.5715718
CCGACGG155150.021.00268612
GCGCACT155700.020.34300434
ACCCGCG149550.020.12642565
CGCGACC158950.019.5748319
TCCCCCG177350.018.8318632
CGACGGC182700.018.12286413
GGCGCGA185950.016.93985417
CCCGACG197050.016.92754411
ACGGCGC199600.016.55307215
CAGTCCG195700.016.4711346
GACGGCG201450.016.36647414
CCCGACC188450.016.19477561
CCGCGCG204500.015.96772167
GGGCGCA202450.015.956552532
CCCCCCC518400.015.9308541
GCGACCC194650.015.89474420
ACCCGCC193200.015.86909523
AGTCCGC205150.015.64416647
CGCACTG205650.015.64018735
Produced by FastQC (version 0.11.5)