FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36295301
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA129600.018.6609579
GTCTAAC50300.018.03291
ACGCACG132600.017.05095552
CGCACGA48050.017.04441828
TACGCAC132150.017.0030851
GTACGCA134050.016.94484550
GTAGCAT146250.016.7279998
CATATGC146200.016.58996812
CCAGTAG141150.016.4899165
CCGGTAC141350.015.74784160
AGTACGC149300.015.68288749
GCCGGTA141950.015.65662159
GCATATG155900.015.33325311
CGGTACA146100.015.307713561
TAAGTAC157950.014.86834347
AGTAGCA165800.014.8611977
CTAAGTA161300.014.42935846
AAGTACG161700.014.37201848
GTCTAAG161900.014.24617544
TTAAGCC161900.014.15970431
Produced by FastQC (version 0.11.5)