FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38380152
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC46500.014.4607961
ACCGTAT42550.013.16073710
CGCACGA45950.012.94859428
TAATCGA27150.012.5056874
CGACCTA24050.012.3711334
GCGATAG82850.012.29305266
GCGCTAA48000.012.24975142
ATAGGCG89150.011.81692262
CGGTCGA29050.011.80698968
GGCGATA87600.011.70638365
CGATAGA88500.011.70597767
CCGTATG45550.011.67925111
TACCGTA53350.011.1526119
TAACACG56300.011.128854
TAGGCGA97600.011.00899963
GTCGAAC29650.010.97785570
TACGCAC29650.010.7418429
CGCTAAG56900.010.7027743
AACACGT61750.010.5994595
TCGAACC34750.010.4747167
Produced by FastQC (version 0.11.5)