FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298988
Sequences flagged as poor quality0
Sequence length300-14998
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4114913.762759709419775No Hit
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16520.5525305363425957No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT13340.4461717527124835No Hit
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12450.41640467175940177No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10470.3501812781783884No Hit
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9400.3143938887179419No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT6110.20435602766666222No Hit
AAGCGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4930.1648895607850482No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG3900.1304400176595716No Hit
AAGCAGTGGTATCAACGCGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3440.11505478480741702No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT3380.11304801530496206No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3230.1080310915488247No Hit
AAGCAGTGGTATCAACGCAAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3060.10234524462520234No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.10167632145771736No Hit
AAGCAGGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3020.10100739829023238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT138800.0413.672031
AGCAGTG146200.0389.70762
AGTGGTA134050.0386.52415
GTGGTAT132100.0381.810036
CAGTGGT140650.0375.201974
GCAGTGG146350.0367.64263
TGGTATC140250.0362.076457
GGTATCA144000.0353.67158
GTATCAA142450.0341.64549
AGTGTAT12000.0221.215385
GTGTATC12350.0214.94616
CATGGTA13000.0200.417374
AAGCGTG7900.0199.124951
AAGCATG15600.0193.799791
CAGTGTA14500.0189.855364
ATGGTAT13150.0188.785425
GCATGGT14650.0181.200323
GTATCAC17150.0174.851159
TGTATCA16850.0173.588077
GCAGTGT19100.0167.294943
Produced by FastQC (version 0.11.5)