FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438327
Sequences flagged as poor quality0
Sequence length300-14986
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4955711.305942823508476Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19660.44852359083515275No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT14930.34061328642771266Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13850.3159741471549779No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12430.28357824181490077Clontech SMART CDS Primer II A (100% over 25bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12330.2812968400303883No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT7040.16061068562967828Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.12456453743438119Clontech SMART CDS Primer II A (100% over 24bp)
AAGCGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4950.11292938833336755Clontech SMART CDS Primer II A (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.10311936065996391No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG4510.10289122048151265Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT4430.10106609905390267Clontech Universal Primer Mix Long (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)