FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004842554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174783
Sequences flagged as poor quality0
Sequence length300-14968
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3006617.201901786787044Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9890.5658445043282241No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT8740.500048631731919Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7070.40450158196163244Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6770.38733744128433545No Hit
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6030.3449992276136695No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3860.22084527671455464Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG3070.17564637293100588Clontech SMART CDS Primer II A (100% over 26bp)
AAGCGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2960.16935285468266364Clontech SMART CDS Primer II A (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.15161657598279007Clontech SMART CDS Primer II A (100% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.14875588586990726No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT2490.14246236762156503Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2140.12243753683138521No Hit
AAGCAGTGGTATCAACGCAAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2060.11786043265077266No Hit
AAGCAGTGGTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1990.1138554664927367No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA1850.10584553417666479Clontech SMART CDS Primer II A (100% over 26bp)
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1820.10412912010893509Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTT1810.10355698208635851Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTAATTTTTTTTTTTTTTTTTTTTTTTTT1750.10012415395089913Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1750.10012415395089913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)