FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004842557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127716
Sequences flagged as poor quality0
Sequence length300-14704
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2025915.862538757869022Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6420.502677816405149No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT6110.4784052115631558Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4480.3507782893294497No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3890.30458204140436596Clontech SMART CDS Primer II A (100% over 25bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2780.217670456324971No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG2560.20044473675968558Clontech SMART CDS Primer II A (100% over 26bp)
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2180.17069122114691973Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCGCCGAGACCGCGTCCG1760.13780575652228383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA1720.13467380751041372Clontech SMART CDS Primer II A (100% over 26bp)
AAGCGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1590.12449497322183595Clontech SMART CDS Primer II A (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC1530.11979704970403082Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT1420.11118418992138808Clontech Universal Primer Mix Long (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)