FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004842558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163777
Sequences flagged as poor quality0
Sequence length300-14933
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1822511.127936157091655Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6900.42130457878700917No Hit
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5600.3419283537981524No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT5580.3407071811060161Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4620.2820908918834757Clontech SMART CDS Primer II A (100% over 25bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3460.2112628757395727No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2230.13616075517319282Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.12578078729003464Clontech SMART CDS Primer II A (100% over 24bp)
AAGCGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2040.12455961459789835Clontech SMART CDS Primer II A (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)