FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304619
Sequences flagged as poor quality0
Sequence length11-48013
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92413.033625611009162Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG42591.3981399715710445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATATATATAGGGTGGTCC40661.3347821376867497Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTTACAACTCCACACTTCAACAACAACAT39501.2967017815697641Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGT38801.2737222563267556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTTACAGGACATGATTAATGAAGTAGATG37331.2254652533164379Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGTGGGGAGGAATGGGGTGGGTTTTGTA25510.8374395556416376Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCTGCAGATGGCGGAAA21510.7061279828244462Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9720.3190871219457749Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGGTTGCAGTGAGCCGAGATCGCACCACTG9410.30891047505244257Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGACCAGGCAGGCCAGAGTGGCCCCACCT7950.2609817509741677Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG7540.2475223147604056Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATATATATAGGGTGGTCCC7410.2432546886438469Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTTACAACTCCACACTTCAACAACAACATG7180.23570427320685838Clontech Universal Primer Mix Long (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGGTGCAAAACTGTAAAGTACCAGATT6930.22749729990578396Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6890.22618418417761202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTTACAGGACATGATTAATGAAGTAGATGC6210.20386121679868952Clontech Universal Primer Mix Long (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGTA5940.1949976856335291Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTATACAAAGTTAAATATAC5210.1710333235943917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAATATATATAGGGTGGTCCC5210.1710333235943917Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCTGCAGATGGCGGAAAT5060.16610913961374701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAGT5060.16610913961374701Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTGTGGGAGGAATGGGGTGGGTTTTGTAT4350.14280133543869555Clontech Universal Primer Mix Long (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT3990.13098329388514832Clontech Universal Primer Mix Long (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTAGAAGGAGGGGTCTTCGTCAAAGAAGT3600.11818041553547218Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGCAAACCATTAATAACA3600.11818041553547218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGCCCGGTAATCGCATAAAACTTAAAACTT3520.11555418407912836Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGGCATGATCTCGGCTTACTGCA3360.11030172116644071Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGCTGCAGATGGCGGAAAT3230.10603409504988197Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)