FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427825
Sequences flagged as poor quality0
Sequence length10-46935
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160283.7463916320925614No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39790.9300531759481097No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.31017355227020393No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.12201250511307193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT62450.0256.091771
AGCAGTG74350.0212.01572
AGTGGTA74950.0189.899175
GTGGTAT73300.0188.432166
CAGTGGT77700.0187.856114
GCAGTGG79050.0186.099963
TGGTATC75700.0181.952677
GGTATCA79100.0172.680598
GTATCAA83450.0163.736689
GCAGTGT15800.067.80363
CAGTGTA14150.066.246274
TATCAAC79000.062.9825639
GTGTATC13300.060.4114766
AGTGTAT14950.056.3032265
TGTATCA17100.049.2241637
TAAGCAG14150.045.9667971
AAGCGTG7250.039.5799331
AAGCATG12750.039.010811
TAGCAGT8450.036.2230532
AAAGCAG22550.034.782361
Produced by FastQC (version 0.11.5)