FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655845
Sequences flagged as poor quality0
Sequence length300-14988
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9742614.855034345005299No Hit
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59080.9008226029016002No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT55410.8448642590856071No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23700.3613658715092743No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT22760.3470332166899191No Hit
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21110.3218748332304127No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT13740.20950072044461726No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG12420.1893740136770121No Hit
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10140.1546097019875123No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA9350.14256417293720317No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT7710.11755826452896645No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC7640.11649093917007829No Hit
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT7120.10856223650405203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT379600.0421.768741
AGTGGTA367750.0383.959565
AGCAGTG416650.0382.07792
GTGGTAT368300.0377.104466
CAGTGGT384900.0373.05414
TGGTATC374650.0365.45737
GCAGTGG401400.0363.666963
GGTATCA379700.0359.754038
GTATCAA395900.0337.697279
GTGTATC46150.0244.257026
AGTGTAT49050.0237.342475
CAGTGTA52750.0226.293764
GCAGTGT57950.0217.45523
TGTATCA54750.0208.137427
GTATCAC34200.0145.371269
AAGCGTG13900.0145.195221
CATGGTA23850.0138.28334
AAGCATG34200.0133.85671
GCATGGT27500.0124.4043053
ATGGTAT26300.0121.6580665
Produced by FastQC (version 0.11.5)