FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707735
Sequences flagged as poor quality0
Sequence length11-59475
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188712.6663934947402628No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29290.4138554685016284No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.12561198753770833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT135900.0311.37411
AGCAGTG163800.0253.631292
AGTGGTA151950.0245.50625
GTGGTAT151550.0243.893266
TGGTATC156300.0234.289117
GGTATCA156950.0231.817938
CAGTGGT166200.0229.73934
GCAGTGG169100.0229.471943
GTATCAA159950.0221.342589
GTGTATC22750.0102.6030046
CAGTGTA27600.091.556244
AGTGTAT25850.091.127015
GCAGTGT32900.084.617943
TGTATCA28350.081.580377
GTATCAC23200.064.62299
TATCAAC157650.064.2604149
AAGCATG25050.054.7125851
TAAGCAG21800.053.045951
TATCACG17450.052.777579
GTGGGTA15150.042.405646
Produced by FastQC (version 0.11.5)