FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721664
Sequences flagged as poor quality0
Sequence length300-14995
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11051915.314467674707345No Hit
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69090.9573707431713374No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT51550.7143213462220646No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT32490.45020951578573964No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26400.36582121319616884No Hit
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26180.36277270308620074No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG18890.2617561635331678No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT16430.22766827775807025No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA13130.18194062610854914No Hit
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11160.15464260376019864No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC11130.1542268978361121No Hit
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT7600.10531216743526073No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT7580.10503503015253635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT429550.0414.801851
AGTGGTA404250.0387.105835
AGCAGTG457100.0387.00042
GTGGTAT401400.0384.27276
CAGTGGT423050.0374.093354
TGGTATC409000.0372.376687
GCAGTGG438600.0366.724463
GGTATCA413450.0366.70148
GTATCAA433650.0341.785039
GTGTATC52350.0259.13786
AGTGTAT57300.0246.63415
CAGTGTA59650.0245.585314
GCAGTGT69200.0227.703543
TGTATCA61950.0222.557687
GTATCAC36650.0158.538189
AAGCGTG14800.0156.373081
AAGCATG36400.0133.2481
CGTGGTA13700.0125.797884
TAAGCAG35650.0122.929561
CATGGTA27950.0116.275434
Produced by FastQC (version 0.11.5)