FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004842575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349806
Sequences flagged as poor quality0
Sequence length300-15000
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5152914.730736465355083Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43301.23782896805658No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT26470.7567051451375906Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16110.46054098557486145No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT11760.3361863432874222Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11230.3210350880202169Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG7120.20354139151415357Clontech SMART CDS Primer II A (100% over 26bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6210.17752697209310303No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT6100.17438237194330572Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA5160.1475103342995832Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC4700.13436018821861262Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT3990.11406322361537538Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTT3500.10005545931173279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)