FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004842576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760116
Sequences flagged as poor quality0
Sequence length10-49884
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT667668.78365933620658Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105171.3836046077177695Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29680.39046671823774265No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.3270553441843087Clontech SMART CDS Primer II A (100% over 24bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.15089802082839987Clontech SMART CDS Primer II A (100% over 23bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9390.1235337764235985No Hit
AGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.105641770466613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)