FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735247
Sequences flagged as poor quality0
Sequence length300-14990
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8620111.72408727951287Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49220.6694348973882247No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT44220.601430539668982Clontech SMART CDS Primer II A (96% over 26bp)
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT26900.36586344452952546Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24670.3355335009867432Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21820.29677101708677495No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT14790.20115689013351976Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG12330.1676987461356524Clontech SMART CDS Primer II A (100% over 26bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9780.13301652369883862No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA9240.1256720530651604Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8570.1165594691307819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)