FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825320
Sequences flagged as poor quality0
Sequence length8-54568
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12240414.831095817379925No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129471.568724858236805No Hit
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52880.6407211748170407No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29230.3541656569573014No Hit
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22690.27492366597198664No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.143823001987108No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG11770.14261135074880046No Hit
AGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.1135317210294189No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC8280.10032472253186642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT338300.0385.65321
AGCAGTG391950.0331.11912
AGTGGTA365350.0330.453435
GTGGTAT367100.0327.555156
TGGTATC371600.0322.340977
GGTATCA373200.0320.425728
CAGTGGT382200.0318.021884
GCAGTGG400900.0304.619423
GTATCAA385350.0303.426579
GTGTATC39150.0178.747076
AGTGTAT44000.0164.061455
CAGTGTA46700.0159.303194
TGTATCA48950.0145.216057
GCAGTGT53000.0144.115833
GTATCAC30900.0105.7660459
AAGCGTG15200.075.52161
AAGCATG28950.073.966421
CATGGTA24150.064.9011844
CGTGGTA11600.064.7041854
ATGGTAT23250.063.6155475
Produced by FastQC (version 0.11.5)